Human MCF7 cells: Mock control vs. siRNA transfected against calreticulin

Transcriptional profiling of human MCF7 cells comparing mock control MCF7cells with MCF7cells transfected by siRNA against calreticulin. The latter was shown to be of having significantly decreased expression of calreticulin followed by significant decrease in migrative and invasive potentials of the MCF7 cells. Goal was to determine the effect of calreticulin knockdown on the global gene expression of MCF7 cells. Two-condition experiment, Mock control MCF7 cells vs. calreticulin siRNA knockdown MCF7 cells. Biological replicates: 3 mock control replicates, 3 transfected replicates.

本数据集针对人乳腺癌MCF7细胞开展转录组谱分析（Transcriptional profiling），比较空白对照组（mock control）MCF7细胞与转染靶向钙网蛋白（calreticulin）的小干扰RNA（siRNA）的MCF7细胞的转录组差异。前期研究证实，该转染组细胞的钙网蛋白表达水平显著下调，同时MCF7细胞的迁移与侵袭能力显著降低。本实验旨在明确钙网蛋白敲低（calreticulin knockdown）对MCF7细胞全基因组基因表达的影响。本实验为双条件对照实验，两组分别为空白对照组MCF7细胞与钙网蛋白siRNA敲低MCF7细胞。生物学重复设置：空白对照组设3次生物学重复，转染组设3次生物学重复。