LncRNAs expression profiling in adipose tissues and during brown adipocyte differentiation

Brown and beige fats generate heat via uncoupled respiration to defend against cold, mechanistically, through the action of a network of transcription factors and cofactors. Here we globally profiled long noncoding RNAs (lncRNAs) gene expression during thermogenic adipocyte formation and identified Brown fat lncRNA 1 (Blnc1) as a novel nuclear lncRNA that promotes brown and beige adipocyte differentiation and function by forming a feedforward regulatory loop with EBF2 to drive adipogenesis toward thermogenic phenotype. LncRNAs expression were measured in BAT and WAT from mice injected saline/CL and during brown adipocyte differentiation with two replicates using Arraystar Mouse LncRNA microarray V2.0

棕色脂肪与米色脂肪可通过解偶联呼吸产热以抵御寒冷，其分子机制依赖于转录因子与辅因子构成的调控网络介导。本研究对产热脂肪细胞形成过程中的长链非编码RNA（long noncoding RNAs, lncRNAs）基因表达开展全基因组表达谱分析，鉴定出棕色脂肪长链非编码RNA1（Brown fat lncRNA 1, Blnc1）作为一种新型核lncRNA。该分子可通过与EBF2形成前馈调控环路，驱动脂肪生成向产热表型方向进行，进而促进棕色与米色脂肪细胞的分化与功能发挥。本研究采用Arraystar小鼠长链非编码RNA芯片V2.0，对注射生理盐水/CL的小鼠的棕色脂肪组织（Brown Adipose Tissue, BAT）与白色脂肪组织（White Adipose Tissue, WAT），以及棕色脂肪细胞分化过程中的lncRNAs表达水平进行检测，实验设置两个重复样本。