Whole-transcriptome sequencing of A549 cells and cisplatin-resistance A549/DPP cells. Whole-transcriptome sequencing of A549 cells and cisplatin-resistance A549/DPP cells

The aim of this study was to investigate the molecular mechanisms underlying cisplatin 14 (DDP) resistance in non-small cell lung cancer (NSCLC) cells by constructing a competing 15 endogenous RNA (ceRNA) network. Overall design: The gene expression profiles of human lung adenocarcinoma DDP-resistant cell line (A549/DDP) and its progenitor (A549) were comparatively evaluated by whole-transcriptome sequencing. The differentially expressed genes (DEGs) were subjected to KEGG pathway analysis. The expression levels of mRNAs involved in several pathways associated with conferring DDP resistance to tumor cells were evaluated. The ceRNA network was constructed based on the mRNA expression levels and the sequencing data of miRNA and lncRNA. Several ceRNA regulatory relationships were validated

本研究旨在通过构建内源竞争RNA（competing endogenous RNA, ceRNA）网络，探究非小细胞肺癌（non-small cell lung cancer, NSCLC）细胞顺铂（cisplatin, DDP）耐药的分子机制。总体设计：本研究通过全转录组测序，对人肺腺癌顺铂耐药细胞系（A549/DDP）及其亲本细胞（A549）的基因表达谱进行比较分析；对差异表达基因（differentially expressed genes, DEGs）开展KEGG通路分析；检测与肿瘤细胞顺铂耐药相关的多条通路中信使RNA（mRNA）的表达水平；基于mRNA表达水平以及微小RNA（miRNA）和长链非编码RNA（lncRNA）的测序数据构建ceRNA调控网络，并对多条ceRNA调控关系进行验证。