Table_5_Microbiome landscape of lesions and adjacent normal mucosal areas in oral lichen planus patient.XLS

The pathogenesis of oral lichen planus (OLP) remains unclear, and microbial dysbiosis has been proposed to play a role in the pathogenesis of OLP. Oral mucosal swabs from 77 OLP patients and 76 healthy subjects were collected. The bacterial community among the OLP lesion, the adjacent normal mucosal, and the oral mucosal surface in healthy people were analyzed by 16S sequencing. The factor of gender and age that may affect the flora distribution of OLP patients were explored. Results indicate no significant difference in microbiota between OLP and the adjacent group. Compared with the healthy group, Neisseria, Haemophilus, Fusobacterium, Porphyromonas, Rothia, Actinomyces, and Capnocytophaga significantly increased in the OLP group. Actinomyces increased in male OLP patients, and the other six bacteria increased in female OLP patients. In female OLP patients, Lautropia and Dialister were positively correlated with age. While in male OLP patients, Moraxella, Porphyromonas, and Fusobacterium were positively correlated with age. Functional enrichment analysis suggested that abnormal energy metabolism related to ATP synthases, abnormal transport and metabolism of glycans, amino acids, and vitamins, and disorders of the local immune microenvironment might exist in OLP lesion.

口腔扁平苔藓（oral lichen planus, OLP）的发病机制目前仍不明确，有研究提出微生物菌群失调（microbial dysbiosis）可能参与了OLP的发病过程。本研究收集了77例OLP患者与76名健康受试者的口腔黏膜拭子样本，采用16S测序技术分析了OLP病灶、邻近正常黏膜以及健康人群口腔黏膜表面的细菌群落组成，并探讨了性别与年龄这两个可能影响OLP患者菌群分布的因素。研究结果显示，OLP组与邻近正常黏膜组的菌群组成无显著差异。与健康对照组相比，OLP组的奈瑟菌属（Neisseria）、嗜血杆菌属（Haemophilus）、梭杆菌属（Fusobacterium）、卟啉单胞菌属（Porphyromonas）、罗氏菌属（Rothia）、放线菌属（Actinomyces）以及二氧化碳噬纤维菌属（Capnocytophaga）的相对丰度显著升高。其中，男性OLP患者体内放线菌属丰度显著上调，而其余六种菌属在女性OLP患者中呈现丰度升高趋势。在女性OLP患者中，劳特罗普菌属（Lautropia）和嗜胨菌属（Dialister）的丰度与年龄呈正相关；而在男性OLP患者中，莫拉菌属（Moraxella）、卟啉单胞菌属及梭杆菌属的丰度与年龄呈正相关。功能富集分析结果提示，OLP病灶中可能存在与ATP合酶（ATP synthases）相关的能量代谢异常、聚糖（glycans）、氨基酸及维生素的转运与代谢紊乱，以及局部免疫微环境失衡等病理特征。