Effect of Chitosan on the Transcriptome of the Fusarium graminearum and Hordeum vulgare L. Pathosystem

Fusarium graminearum (Fg) is a cosmopolitan fungal pathogen that causes significant losses in cereal production worldwide, both through yield reduction and grain contamination with mycotoxins. Barley (Hordeum vulgare L.) is one of the most important crop plants globally. In barley, Fg causes a disease known as Fusarium head blight (FHB). Chitosan, a natural biopolymer abundant in the environment, has proven antifungal properties and acts as a plant immunity elicitor. In our project, we sequenced the Hordeum vulgare L. (Golden Promise) and Fusarium graminearum pathosystem following chitosan treatment. The study is based on four treatments: Hv_mock (non-treated barley), Hv_CS (barley treated with chitosan), Hv_Fg (barley inoculated with F. graminearum), and Hv_Fg_CS (barley inoculated with F. graminearum and treated with chitosan). Three biological replicates were sequenced for each treatment

禾谷镰孢菌（Fusarium graminearum，简称Fg）是一种全球性分布的真菌病原菌，可通过降低产量与使谷物被真菌毒素污染两种途径，对全球谷物生产造成严重损失。大麦（Hordeum vulgare L.）是全球最重要的农作物之一。在大麦上，Fg可引发一种名为赤霉病（Fusarium head blight，简称FHB）的病害。壳聚糖（Chitosan）是一种环境中广泛存在的天然生物聚合物，已被证实具有抗真菌活性，同时可作为植物免疫激发子。本研究针对经壳聚糖处理后的大麦（Hordeum vulgare L. cv. Golden Promise）与禾谷镰孢菌的病害互作系统开展了测序工作。本研究共设置四组处理：Hv_mock（未处理大麦对照组）、Hv_CS（经壳聚糖处理的大麦）、Hv_Fg（接种禾谷镰孢菌的大麦）以及Hv_Fg_CS（接种禾谷镰孢菌并经壳聚糖处理的大麦）。每组处理均设置三次生物学重复并完成测序。