Data for the Blood Proteoform Atlas:DS-47:Monocyte:Purchased from STC

Human biology is tightly linked to proteins, yet most measurements do not precisely determine their full sequence and post-translational modifications. Here, we present the primary structures of 30,000 unique proteoforms expressed from 1,690 human genes across 21 cell types and plasma from human blood and bone marrow compiled in the Blood Proteoform Atlas (BPA). Our results indicate that while a given protein can be expressed across multiple cell types, the proteoform functions as a more specific indicator of differentiation. These results provide a better biochemical description of protein-level biology expressed through gene transcription and translation. We demonstrate the utility of the BPA by focusing on cell- and proteoform-specific signatures within 58 liver transplant recipients having healthy graft function or undergoing acute organ rejection or dysfunction.

人体生物学与蛋白质紧密关联，但当前绝大多数检测手段无法精准测定蛋白质的完整序列与翻译后修饰（post-translational modifications）。本研究构建了血液蛋白质形图谱（Blood Proteoform Atlas, BPA），其中收录了来自21种细胞类型以及人体血液、骨髓来源血浆中，由1,690个人类基因表达的30,000种独特蛋白质形（proteoform）的一级结构。研究结果表明，尽管同一蛋白质可在多种细胞类型中表达，但蛋白质形可作为细胞分化更具特异性的标志物。上述结果为通过基因转录与翻译过程所表达的蛋白质层面生物学特性提供了更完善的生化描述。本研究以58名肝移植受者为研究对象，聚焦其移植物功能正常、发生急性器官排斥或功能障碍时的细胞特异性与蛋白质形特异性特征，以此验证了BPA的应用价值。