Methyl-CpG-binding domain (MBD) sequencing of 15 primary stage 4S neuroblastoma tumors

The DNA methylome of 15 primary stage 4S neuroblastoma tumors is profiled by enrichment with a methyl-CpG-binding domain (MBD) and massively parallel sequencing DNA of 15 primary stage 4S tumors is sheared (fragments of ± 200 bp), followed by MBD-based (MethylCap kit of Diagenode) enrichement, library preparation and multiplexing. Both input DNA and captured DNA were sequenced paired-end on Illumina Hiseq2000

本数据集针对15例原发性4S期神经母细胞瘤（neuroblastoma）组织的DNA甲基化组（DNA methylome），采用甲基-CpG结合结构域（methyl-CpG-binding domain, MBD）富集结合大规模平行测序技术开展谱型分析：将15例该类肿瘤的基因组DNA剪切为长度约200 bp的片段，随后使用基于该结构域的Diagenode公司MethylCap试剂盒完成富集流程，继而进行文库制备与多重复合。最终，起始DNA与捕获得到的DNA均在Illumina Hiseq2000测序平台上进行双端测序。