Gene expression profiling of preneoplastic Lgr5-creERT2/PIK3CAH1047R mammary subsets. Mus musculus

This study examined the effect of mutant PIK3CAH1047R expression in mammary subsets of preneoplastic mammary glands from Lgr5-creERT2/PIK3CA H1047R mice Overall design: Mammary cell subpopulations were isolated from Lgr5-creERT2/PIK3CA H1047R and Lgr5-creERT2 control animals 4 weeks after activation of PIK3CA H1047R transgene expression by Tamoxifen injection. Pooled mammary glands of 2-3 estrus-synchronized mice per genotype were sorted in 3 independent sortings and used for microarray analysis (24 samples in total).

本研究探究了Lgr5-creERT2/PIK3CA H1047R小鼠癌前乳腺腺体的乳腺细胞亚群中，突变型PIK3CAH1047R（mutant PIK3CAH1047R）的表达效应。
总体设计：通过他莫昔芬（Tamoxifen）注射激活PIK3CA H1047R转基因表达4周后，从Lgr5-creERT2/PIK3CA H1047R与Lgr5-creERT2对照小鼠体内分离乳腺细胞亚群。每个基因型取2-3只发情同步化小鼠的乳腺腺体混合，经3次独立分选后用于微阵列分析（microarray analysis），共计24个样本。