MBD-Seq was applied to study the CpG methylation map of rhabdomyosarcoma cells after DiPRO1/ZNF555 inhibition

The methylation enrichment was determined by MIRA-sequencing after MBD2-capture using the MethylCollector™ Ultra Kit (Active Motif) performed on DNA samples derived from rhabdomyosarcoma cells (RMS) TE671 cells. The cells expressing shRNA targeting ZNF555 (shZNF555) were compared to the control cells expressing non-targeting shRNA (shCtl). The signals of methylation-enriched DNA (ENR) were normalised to non-enriched input DNA (INP).

本数据集的实验以源自横纹肌肉瘤（rhabdomyosarcoma, RMS）细胞系TE671的DNA样本为材料，经MBD2蛋白捕获（MBD2-capture）后，使用Active Motif公司的MethylCollector™超试剂盒（MethylCollector™ Ultra Kit），通过MIRA测序（MIRA-sequencing）测定甲基化富集水平。将表达靶向ZNF555的短发夹RNA（shRNA，shZNF555）的细胞，与表达非靶向短发夹RNA（shCtl）的对照细胞进行比较。将甲基化富集DNA（ENR）的信号标准化至非富集输入DNA（INP）的信号。