RNA-Seq of roseoflavin producer Streptomyces davaonensis reveals transcription of unusual high number of riboflavin biosynthetic genes (rib genes) in exponential and stationary growth phase. RNA-Seq of roseoflavin producer Streptomyces davaonensis reveals transcription of unusual high number of riboflavin biosynthetic genes (rib genes) in exponential and stationary growth phase

RNA-Seq of roseoflavin producer Streptomyces davaonensis reveals transcription of unusual high number of riboflavin biosynthetic genes (rib genes) in exponential and stationary growth phase. In this study mRNA from three biological replicates of Streptomyces davaonensis harvested in exp. (after 18 h of growth) and stationary growth phase (after 48 h of growth) were sequenced after Illumina Ribozero treatment via Illumina TruSeq stranded mRNA libs on Illumina HiSeq1500. Moreover primary 5′-end-enriched cDNA libraries were prepared according to Peifer-Sancar et al. (2013) and sequenced on Illumina MiSeq system.

针对玫瑰黄素产生菌达沃斯链霉菌（Streptomyces davaonensis）的RNA测序分析显示，该菌在指数生长期与稳定生长期中转录得到异常大量的核黄素生物合成基因（rib基因）。本研究采集了达沃斯链霉菌的三次生物学重复样本，分别获取其指数生长期（培养18小时后）与稳定生长期（培养48小时后）的mRNA，经Illumina Ribozero处理后，采用Illumina TruSeq链特异性mRNA文库构建策略，在Illumina HiSeq1500测序平台完成测序。此外，本研究参照Peifer-Sancar等（2013）的方法制备了初级5′端富集cDNA文库，并在Illumina MiSeq测序系统上完成测序。