RNA-Induced Silencing Complex-associated RNA in MRC-5 cells transfected with hcmv-miR-UL22A-5p. Homo sapiens

Investigation of mRNA targets of the human cytomegalovirus miRNA UL22A-5p Overall design: One 4-plex chip was hybridized with cDNA originating from either total RNA or from AGO2-associated RNA (i.e. RISC-associated RNA) from fibroblasts transfected 48 hours earlier with hcmv-miR-UL22A-5p mimic or with a negative control siRNA

人巨细胞病毒miRNA UL22A-5p的mRNA靶标研究
实验设计概况：本实验使用1张4联芯片，与四类互补DNA（complementary DNA, cDNA）进行杂交。这四类cDNA分别来源于转染hcmv-miR-UL22A-5p模拟物或阴性对照小干扰RNA（small interfering RNA, siRNA）48小时后的成纤维细胞：一类为该细胞总RNA反转录所得的cDNA，另一类为该细胞中与AGO2蛋白（Argonaute 2）结合的RNA（即RNA诱导沉默复合体（RNA-induced silencing complex, RISC）结合RNA）反转录所得的cDNA。