Original blots

To investigate the roles of various variablesin the induction of adjuvant-induced arthritis (AIA) inthe outbred Sprague-Dawley (SD) rats, and further characterizeits arthritic features by comprehensive examinations.Methods: The roles of different preparative techniques,inoculation routes and doses of Mycobacterium tuberculosis(MT) suspension as well as the sex preference in theinduction of AIA were comparatively studied using clinicalassessment. The hind paws of animals were analyzed by radiologicaland histological examinations. The serum levelsof cytokines interleukin (IL)-1b, IL-6, and tumor necrosisfactor (TNF)-a were determined by ELISA.

为探究不同变量在远交系Sprague-Dawley（SD）大鼠佐剂性关节炎（adjuvant-induced arthritis, AIA）造模中的作用，并通过全面检测进一步明确其关节炎特征。方法：本研究通过临床评估，对比考察结核分枝杆菌（Mycobacterium tuberculosis, MT）混悬液的不同制备工艺、接种途径与接种剂量，以及性别偏好对AIA造模的影响；对受试动物的后爪开展影像学与组织病理学检测分析；采用酶联免疫吸附试验（ELISA）检测血清中白细胞介素（IL）-1β、IL-6以及肿瘤坏死因子（TNF）-α的水平。