Lens epithelial cell transcriptome reprogramming at 24 hours post cataract surgery (PCS) in wild type (WT) and β8 integrin Conditional Knockout (β8ITGcKO) mice.

This trancriptome analysis reveals the first detailed analysis on how the remnant lens epithelial cells (LECs) greatly reprogram their transcriptome by 24 hours post cataract surgery (PCS) and the dependence of this phenomenom on endogenous β8 integrin gene expression. Living mice of both WT and β8ITGcKO were subjected to lens fiber cell removal leaving behind the lens epithelium and capsule to model human cataract surgery. NovaSeq 6000, S2 100PE transcriptome profiling was performed in biological triplicate on remnant LECs isolated either immediately after surgery or 24 hours later in order to ascertain the response of lens epithelial cells to cataract suregery with the goal of understanding the role of αVβ8 integrin in posterior capsular opacification (PCO) pathogenesis.

本转录组分析首次详细解析了白内障手术后（PCS）24小时内，残留晶状体上皮细胞（lens epithelial cells，简称LECs）如何大规模重编程其转录组，以及该现象对内源性β8整合素基因表达的依赖性。本研究以野生型（WT）和β8ITGcKO基因敲除小鼠为实验对象，通过摘除晶状体纤维细胞、保留晶状体上皮与囊膜的方式模拟人类白内障手术模型。分别于术后即刻及术后24小时分离残留LECs，采用NovaSeq 6000测序平台S2流动槽100bp双端测序进行转录组分析，设置三次生物学重复，以此探究晶状体上皮细胞对白内障手术的应答反应，最终明确αVβ8整合素在后发性白内障（posterior capsular opacification，简称PCO）发病机制中的作用。