PAF induces sudden death in Swiss albino mice.

Swiss albino mice were divided into 9 groups containing 6 animals each. The indicated amounts of WEB-2086 or BN-52021 were aliquoted from a stock in DMSO, brought up to a total volume of 0.5 mL in PBS, and injected intraperitoneally into animals 30 min before PAF (5 μg/mouse) was injected. Animals that received rPAF-AH (25 μg/mouse) were intraperitoneally injected with it 30 min before being injected with PAF (5 μg/mouse), as were the animals that received the vehicle for rPAF-AH (sodium citrate, sucrose, pluronic, and Tween-80). All the animals injected with PAF alone (5 μg/mouse) died within 15–20 min. In contrast, all the animals that received either a PAF-R antagonist or rPAF-AH 30 min before being injected with a lethal dose of PAF survived. All animals were monitored for survival for up to 6 days. The results are representative of 3 individual trials.

将瑞士白化小鼠分为9组，每组各6只。将指定剂量的WEB-2086或BN-52021从二甲基亚砜（DMSO）储备液中定量移取，用磷酸盐缓冲液（PBS）定容至总体积0.5 mL，于血小板活化因子（PAF，5 μg/只）注射前30分钟对小鼠进行腹腔注射。接受重组血小板活化因子乙酰水解酶（rPAF-AH，25 μg/只）的小鼠，以及接受rPAF-AH赋形剂（柠檬酸钠、蔗糖、普朗尼克（Pluronic）、吐温-80（Tween-80））的小鼠，均于PAF（5 μg/只）注射前30分钟接受腹腔注射。仅注射PAF（5 μg/只）的全部小鼠均在15~20分钟内死亡。与之相对，在致死剂量PAF注射前30分钟给予PAF受体拮抗剂或rPAF-AH的全部小鼠均存活。所有小鼠均被监测最长6天的存活情况，本实验结果为3次独立试验的代表性结果。