Phosphoglycerate dehydrogenase causes electron transport chain defect via impaired heme synthesis: RNA sequencing.

Given the prominent angiogenic impairment in PHGDHECKO mice and the proliferation defect of PHGDHKD ECs, which could not fully be explained by a decreased one carbon metabolism pool, together with the observed impairment of mitochondrial homeostasis and reduced respiration in PHGDHKD ECs, we performed an RNA-seq analysis of control and PHGDHKD ECs.

鉴于PHGDHECKO小鼠存在显著的血管生成损伤，且PHGDHKD内皮细胞（endothelial cells, ECs）呈现增殖缺陷，上述表型无法仅通过一碳代谢库的减少得到完全解释；加之我们在PHGDHKD内皮细胞中观察到线粒体稳态受损与呼吸功能降低，因此我们对对照组及PHGDHKD内皮细胞开展了RNA测序（RNA-seq）分析。