Retroviral overexpression or siRNA mediated knockdown of FOXP1 in DLBCL cell lines and primary human B cells

To provide insight into the role of and target genes of the transcription factor FOXP1 in mature human B cells and in B cell non-Hodkgin lymhomas, we performed gene expression microarray studies, upon ectopic overexpression or silencing of FOXP1 in these cells. human memory B cells from 2 separate donors were transduced with LZRS-FOXP1-IRES-YFP or LZRS-IRES-YFP (negative control); DLBCL cell lines OCI-Ly1, OCI-Ly7, and OCI-Ly10 were transduced with LZRS-FOXP1-IRES-YFP or LZRS-IRES-YFP (negative control); DLBCL cell lines OCI-Ly1, OCI-Ly7, and OCI-Ly10 were transiently transfected with siRNA targeting FOXP1 or sigenome non-targeting siRNA (negative control), using the Lonza nucleofection system.

为解析转录因子FOXP1在成熟人类B细胞及B细胞非霍奇金淋巴瘤中的作用与靶基因，我们针对该类细胞中FOXP1的异位过表达或基因沉默模型开展了基因表达微阵列研究：从2名独立供者分离的人类记忆B细胞，分别以LZRS-FOXP1-IRES-YFP载体或LZRS-IRES-YFP载体（阴性对照）进行转导；弥漫大B细胞淋巴瘤（DLBCL）细胞系OCI-Ly1、OCI-Ly7及OCI-Ly10，同样以LZRS-FOXP1-IRES-YFP载体或LZRS-IRES-YFP载体（阴性对照）进行转导；采用Lonza核转染系统，将靶向FOXP1的小干扰RNA（siRNA）或非靶向阴性对照siRNA，瞬时转染至上述DLBCL细胞系中。