Spatio-temporal transcriptome maps in the early embryos of Halocynthia roretzi

The ascidia Halocynthia roretzi (Phylum: Chordata, Subphylum: Tunicata, Class: Ascidiacea) have been used as model species in development biology for over a century. It offers attractive experimental features, including a compact genome, invariant embryonic cell lineages, small embryonic cell number, and translucent embryos, which allow the description of developmental processes with a cellular level of resolution. Staged RNA-seq data has been already deposited in DDBJ (accessiton number DRA005714 and DRA005711). In the present study, we sequenced genome of H. roretzi of a southwestern Japanese population using RNA-Seq data. These RNA-Seq data covers expressions in 8 cell and 110 cell embryos of Animal(A), Vegetal(V) and whole-embryo regions. These genome assembly, transcript assembly, and transcript models are incorporated into the ANISEED (https://www.aniseed.cnrs.fr/) for genome browsing and blast searches. The genome and transcriptome resources will be useful datasets for developmental biology, evolutionary biology and molecular ecology using this model organism. Overall design: Examination of different expressions in 8 cell and 110 cell embryos of Animal(A), Vegetal(V) and whole-embryo regions

柄海鞘（Halocynthia roretzi，门：脊索动物门（Chordata）、亚门：尾索动物亚门（Tunicata）、纲：海鞘纲（Ascidiacea））一个多世纪以来一直被用作发育生物学研究的经典模式生物。该物种具备诸多极具吸引力的实验特性：基因组紧凑、胚胎细胞谱系恒定、胚胎细胞总量较少且胚胎通体透明，可实现细胞分辨率水平的发育过程观测与描述。按发育阶段分期的RNA测序（RNA-seq）数据已提交至日本DNA数据银行（DDBJ），登录号为DRA005714与DRA005711。本研究利用RNA-seq数据，对日本西南部种群的柄海鞘基因组进行了测序。本次所用的RNA-seq数据涵盖了8细胞期、110细胞期胚胎的动物极（A）、植物极（V）以及全胚区域的基因表达谱。本研究获得的基因组组装结果、转录组组装结果以及转录本模型已整合至ANISEED数据库（https://www.aniseed.cnrs.fr/），可供基因组浏览与BLAST序列比对检索使用。该基因组与转录组资源可作为该模式生物相关研究的优质数据集，应用于发育生物学、进化生物学及分子生态学领域。实验设计：对8细胞期、110细胞期胚胎的动物极（A）、植物极（V）及全胚区域的基因表达差异进行检测。