HNP-1 potentiates HIV-1 inhibition by peptides targeting the gp41 N-HR domain.

Fusion experiments between HXB2 (A, C) and BaL (B, D) pseudoviruses and TZM-bl cells were performed as described in the legend to Figure 2, using varied concentrations of C34 (A, B) or N36mut(e,g) (C, D) in the presence (red symbols) or in absence (black symbols) of 7.3 µM HNP-1 in HBSS supplemented with 10% human serum. Data points are means and SEM from a representative triplicate experiment; the solid lines are obtained by curve-fitting, as described above. See Table 2 for the respective IC50 values.

本研究依照图2图例所述的实验方法，开展了HXB2（A、C组）与BaL（B、D组）假病毒、TZM-bl细胞的融合实验。实验体系中添加了不同浓度的C34（A、B组）或N36mut(e,g)（C、D组），并分为添加7.3 μM HNP-1（以红色符号标记）与不添加7.3 μM HNP-1（以黑色符号标记）两个组别，所用缓冲液为添加10%人血清的汉克斯平衡盐溶液（HBSS）。本实验为单次代表性三次重复实验，数据点均为三次重复的平均值与标准误（Standard Error of the Mean，SEM）；拟合得到的实线采用前文所述的曲线拟合方法生成。相关半数抑制浓度（IC50）值详见表2。