ChIP-seq for histone modifications during MCF10A-ER-Src cell transformation

We performed ChIP-seq for histone modifications to map chromatin status and dynamics during MCF10A-ER-Src cell transformation. ChIP-seq for H3K27ac, H3K4me3, H3K4me1, H3K36me3, H3K27me3 and H3K9me3 at 0, 2, 12, 24 and 36 hr during MCF10A-ER-Src cell transformation

本研究针对组蛋白修饰开展染色质免疫共沉淀测序（ChIP-seq），以解析MCF10A-ER-Src细胞转化过程中的染色质状态与动态变化。在MCF10A-ER-Src细胞转化的0、2、12、24及36小时时间点，分别针对H3K27ac、H3K4me3、H3K4me1、H3K36me3、H3K27me3与H3K9me3这六种组蛋白修饰完成了ChIP-seq实验。