Microbiome response to cadmium and copper in Steatoda grossa (Theridiidae) spiders

The dataset associated with the publication titled "Microbiome Response to Cadmium and Copper Ingestion in the Spider Steatoda grossa (Theridiidae): Short-Term and Long-Term Effects of Metal Intoxication” was funded by NCN.The project presents data on the quantitative and qualitative changes in the microbiome of the opisthosoma of Steatoda grossa (Theridiidae) spiders in response to copper and cadmium exposure through prey (Drosophila hydei).The collected data cover:Metal concentrations in spider bodies after short-term (4 weeks) and long-term (12 months) exposure.The composition of the opisthosoma microbiome in control individuals.Characteristics of changes in the microbiome’s qualitative and quantitative structure due to the presence of metals in the diet, depending on the duration of exposure.Analysis of species diversity in the microbiome in relation to the type of metal used for diet contamination.DNA was isolated using the QIAamp DNA Microbiome Kit (Qiagen). Genomic DNA (gDNA) was further purified with the Monarch Genomic DNA Purification Kit (NEB). Its quality was checked using D1000 Screen Tapes and the Tape Station 2200 (Agilent). DNA libraries were prepared following Illumina’s 16S Metagenomic Sequencing Library Preparation protocol. Amplification was performed with KAPA HiFi HotStart ReadyMix (Roche) on a SureCycler 8800 (Agilent). Library quality and quantity were assessed with D1000 Screen Tapes (Tape Station 2200, Agilent) and Qubit dsDNA High Sensitivity Kit (Thermo Fisher). Libraries were pooled in equimolar ratios and sequenced (2 × 250) on the Illumina MiSeq platform. Raw sequence data are available in the NCBI SRA database under BioProject accession number PRJNA1180465.The sequences were assembled into contigs and processed using Mothur 1.44 software. They were filtered for size, quality, homopolymer length, and nucleotide ambiguity, then aligned to the SILVA 1.38 database.

本数据集与题为《球蛛科蜘蛛Steatoda grossa微生物组对镉和铜摄入的响应：金属中毒的短期与长期效应》的出版物相关，由波兰国家科学中心（NCN）资助。该项目呈现了Steatoda grossa（球蛛科）蜘蛛通过猎物（海德氏果蝇Drosophila hydei）暴露于铜和镉后，其腹部微生物组在定量与定性层面变化的数据。收集的数据涵盖：短期（4周）与长期（12个月）暴露后蜘蛛体内的金属浓度；对照组个体的腹部微生物组组成；饮食中金属存在导致的微生物组定性与定量结构变化特征（取决于暴露持续时间）；以及与饮食污染所用金属类型相关的微生物组物种多样性分析。

DNA分离采用QIAamp DNA Microbiome Kit（Qiagen），基因组DNA（gDNA）通过Monarch Genomic DNA Purification Kit（NEB）进一步纯化，其质量利用D1000 Screen Tapes和Tape Station 2200（Agilent）检测。DNA文库制备遵循Illumina的16S宏基因组测序文库制备 protocol，扩增使用KAPA HiFi HotStart ReadyMix（Roche）在SureCycler 8800（Agilent）上进行。文库质量与数量通过D1000 Screen Tapes（Tape Station 2200，Agilent）和Qubit dsDNA High Sensitivity Kit（Thermo Fisher）评估。文库按等摩尔比混合后，在Illumina MiSeq平台上进行2×250bp测序。原始序列数据可在NCBI SRA数据库中获取，BioProject登录号为PRJNA1180465。序列被组装为contigs，并使用Mothur 1.44软件处理，过滤条件包括片段大小、质量、均聚物长度及核苷酸歧义性，随后与SILVA 1.38数据库进行比对。