Research data supporting "Independent effects on cellular and humoral immune responses underlie genotype-by-genotype interactions between Drosophila and parasitoids"

Datasets and scripts to generate each figure panel are divided in separate folders. Scripts generate and save each figure as a PDF file. Statistics are generated at the end of each script. Folders: Fig1A – The encapsulation phenotype is recorded for individual larva per treatment and genotype. Fig1B – The melanization phenotype is recorded for individual larva per treatment and genotype. Fig2 – The number of plasmatocytes and lamellocytes is recorded from pool of 6 larvae per treatment and genotype. Fig3A – Gene expression is recorded as raw Ct values from qPCR for housekeeping gene and test gene per treatment and genotype. Fig3B – Phenoloxidase activity is recorded as raw absorbance value per time point, treatment and genotype. FigS1 – The encapsulation phenotype is recorded per vial with 40 transferred larvae before infection.

本数据集及用于生成各图面板的脚本均划分至独立文件夹中。各脚本可生成对应图表并将其保存为PDF格式文件，且会在各脚本的末尾输出统计结果。
文件夹详情如下：
Fig1A：针对各处理组与基因型，记录单条幼虫的包囊表型（encapsulation phenotype）；
Fig1B：针对各处理组与基因型，记录单条幼虫的黑化表型（melanization phenotype）；
Fig2：针对各处理组与基因型，从每6条幼虫的混合样本中统计浆血细胞（plasmatocytes）与层黏血细胞（lamellocytes）的数量；
Fig3A：针对各处理组与基因型，记录看家基因与靶基因的qPCR原始Ct值，以此表征基因表达水平；
Fig3B：针对各时间点、处理组与基因型，记录酚氧化酶（phenoloxidase）活性的原始吸光度值；
FigS1：在感染前，对每瓶中转移的40条幼虫记录其包囊表型（encapsulation phenotype）。