Sequencing variants on heartbeats cells

This dataset is for a quantitative study which include figures, graphs and tables to show unexplained death and the KCNQ1 gene potassium channel found in heart cells. There are figures describing previous literature and explains the concepts. The graphs show the results of polymerase chain reaction (PCR) curves that were used to optimise the primer sets and the results of high resolution melting (HRM) analysis of the PCR results, which was used to sort the dataset into potential variants for sequencing. The tables describe the conditions for the PCR reactions and the components that were used for the reactions, including a table on the concentration of the DNA samples extracted. Some tables also showed the full expent of what the research attempt to do to optimise reactions by detailing the results from the different annealing temperatures. Other tables descibes all the cases that were sequenced and variants that were found. A separete table also has the details of the primers used for the PCR reactions.

本数据集服务于一项定量研究，其中包含各类图件、图表与表格，用于展示不明原因死亡相关数据以及心肌细胞中存在的KCNQ1基因钾离子通道（KCNQ1 gene potassium channel）。部分图件用于阐释既往研究文献，并对相关概念进行解释。图表部分展示了用于优化引物组的聚合酶链式反应（polymerase chain reaction, PCR）曲线结果，以及基于该PCR结果开展的高分辨率熔解分析（high resolution melting, HRM）结果——该分析用于将数据集划分为可用于后续测序的潜在变异样本。表格部分涵盖了PCR反应的反应条件与反应所用组分，其中一张表格记录了提取的DNA样本浓度信息。部分表格还通过详述不同退火温度下的实验结果，完整呈现了本研究为优化反应体系所开展的全部探索。其余表格则详细记录了所有完成测序的样本案例，以及所发现的基因变异情况。另有一张单独表格列明了PCR反应所用引物的全部详细信息。