RNA-seq analysis of gene expression in male and female mouse liver

Gene expression in adult male and female mouse liver was assayed by RNA-seq, as part of a study on chromatin states in male and female mouse and their role in sex-biased liver gene expression (A Sugathan and DJ Waxman (2013) Mol Cell Biol. 33:3594-3610. doi: 10.1128/MCB.00280-13). Total liver RNA was prepared from 12 individual male and 12 individual female mice. Four RNA pools, comprised of RNA isolated from 6 individual male or female livers (2 pooled biological replicates for each sex) were then prepared and used for RNA-seq.

本数据集针对成年雄性与雌性小鼠肝脏的基因表达水平开展RNA测序（RNA-seq）分析，相关研究隶属于一项探究雌雄小鼠染色质状态及其在性别偏好性肝脏基因表达中调控作用的课题（A Sugathan与DJ Waxman (2013) Mol Cell Biol. 33:3594-3610. doi: 10.1128/MCB.00280-13）。实验从12只独立雄性小鼠与12只独立雌性小鼠体内提取总肝脏RNA。随后将每6只同性别小鼠的肝脏RNA进行混合，构建得到4个RNA混合样本（每个性别设置2个生物学重复混合池），所有混合样本均用于RNA-seq测序。