Calibration sausages Metagenome. food metagenome

Within this project we extracted and sequenced total DNA of so called sausage calibrators (sausages prepared under laboratory conditions with known compositions of different mammalian and avian species). The data was used to develop an calibrate a new bioinformatic pipeline namely AFS (All Food Sequencing) that is feasible to accurately determine species composition in food samples in a qualitative and quantitative way by a readmapping/-counting approach.

本项目中，我们对所谓的香肠校准品（sausage calibrators）的总DNA进行了提取与测序；该校准品指在实验室条件下制备、成分明确的不同哺乳类与鸟类物种混合制成的香肠。所得数据被用于开发并校准一款全新的生物信息学流程AFS（All Food Sequencing，全食品测序），该流程可通过读段比对/计数（readmapping/-counting）法，实现对食品样本中物种组成的定性与定量精准鉴定。