Biological effect of chronic mistranslation in mammalian cells

Analysis of HEK293 cells lines expressing mutant ribosomal protein RPS2 (human A226Y). RPS2 A226Y mutation has been shown to cause misreading and readthrough. Results provide insight into the response to chronic mistranslation in mammalian cells. mRNA profiles of RPS2 wild-type and RPS2 A226Y mutant HEK293 cell culture samples generated by deep sequencing.

本数据集针对表达人源A226Y突变型核糖体蛋白RPS2的HEK293细胞系展开分析。已有研究证实，RPS2 A226Y突变可引发翻译错读与通读现象。本研究结果为解析哺乳动物细胞内慢性错译的细胞应答机制提供了关键参考。本数据集通过高通量测序（deep sequencing）获取了RPS2野生型与RPS2 A226Y突变型HEK293细胞培养样本的mRNA表达谱。