Spindle-localized mRNA translation mediated by CPEB1 and CPEB4 controls mitotic progression. Homo sapiens

Here we show sequential functions of CPEB1 and CPEB4 in the localization of repressed mRNAs to the mitotic spindle and their subsequent phase -specific activation to promote cell phase transitions and correct chromosome segregation. Overall design: RNA-immunoprecipitation sequencing of CPEB1 and CPEB4-associated mRNAs in Hela S3 cells.

本研究阐明了CPEB1与CPEB4的时序性功能：二者可将受抑制的mRNA定位至有丝分裂纺锤体，并通过后续的细胞周期阶段特异性激活过程，促进细胞周期相变与染色体的正确分离。整体实验设计：对HeLa S3细胞中与CPEB1、CPEB4结合的mRNA开展RNA免疫共沉淀测序（RNA-immunoprecipitation sequencing）。