Proteomic profiling reveals the molecular changes of insomnia patients

Insomnia is an economic burden and public health problem. This study aimed to explore potential biological pathways and protein networks for insomnia characterized by wakefulness after sleep. Proteomics analysis was performed in the insomnia group with wakefulness and the control group. The differentially expressed proteins (DEPs) were enriched, then hub proteins were identified by protein-protein interaction (PPI) network and verified by parallel reaction monitoring (PRM). Compared with the control group, the sleep time and efficiency of insomnia patients were decreased, awakening time and numbers after sleep onset were significantly increased (P < 0.001).

失眠既是一项经济负担，也是一类公共卫生问题。本研究旨在探索以睡眠后觉醒为特征的失眠的潜在生物学通路与蛋白质网络。研究对伴睡眠后觉醒症状的失眠组与对照组开展蛋白质组学分析，对差异表达蛋白（differentially expressed proteins, DEPs）进行富集分析，随后通过蛋白质-蛋白质相互作用（protein-protein interaction, PPI）网络筛选枢纽蛋白，并采用平行反应监测（parallel reaction monitoring, PRM）对枢纽蛋白进行验证。与对照组相比，失眠患者的睡眠时间与睡眠效率均显著降低，睡眠起始后的觉醒时长与觉醒次数均明显升高（P < 0.001）。