Effect of Nitric oxide on heart development and its transcriptome analysis.

Scope: To understand the effect of nitric oxide on cardiogenesis in reference to first heart beat. Overall design: Methods: The trancriptome sequencing was performed using Illumina HiSeq 2500 ® platform. The sequence reads were aligned to the reference genome of chicken (Galgal4) downloaded from Ensembl Release 81 database using STAR (version 2.4.2a ). Expression estimation carried out using cufflinks program (version 2.2.1).Differential expression analysis carried using the cuffdiff program in cufflinks(version 2.2.1). Heat maps were generated for gene expression analysis using R statistical software.

研究范围：旨在探究一氧化氮对心脏发生的影响，以首次心跳作为参照指标。整体实验设计：实验方法如下：采用Illumina HiSeq 2500 ® 平台开展转录组测序；将测序读段比对至从Ensembl Release 81数据库下载的鸡参考基因组（Galgal4），比对工具为STAR（版本2.4.2a）；使用cufflinks软件（版本2.2.1）进行表达量估算；利用cufflinks套件中的cuffdiff程序（版本2.2.1）开展差异表达分析；采用R统计软件绘制用于基因表达分析的热图。