DataSheet_1_PCDH9 suppresses melanoma proliferation and cell migration.zip

BackgroundMelanoma has dramatically increased during last 30 years with low 5-year survival and prognosis rate.
MethodsMelanoma cells (A375 and G361) were chosen as the in vitro model. The immunohistochemical (IHC) analysis and bioinformatics mining exhibited the suppression of PCDH9 on melanoma. The interference and overexpression of PCDH9 were infected by lentivirus. The effects of PCDH9 on melanoma cells were assessed in terms of alteration of PCDH9 such as cell viability, apoptosis, cell cycle, and wound-healing assay. Moreover, expressions of PCDH9 with other genes (MMP2, MMP9, CCND1, and RAC1) were also assessed by PCR.
ResultsThe alteration of PCDH9 has a negative correlation with MMP2, MMP9, and RAC1 but had a positive correlation with CCND1 (Cyclin D1) and apoptosis. Increase of PCDH9 could suppress melanoma cells and inhibit migration but not exert significant effects on cell cycle. IHC showed lower PCDH9 expression in melanoma tissue with main expression in cytoplasm.
ConclusionOverexpressed PCDH9 suppressed melanoma cells, and PCDH9 can be considered as an independent prognostic factor for melanoma; even re-expression of PCDH9 can serve as a potential therapeutic strategy for melanoma treatment.

背景：过去三十年间，黑色素瘤发病率显著上升，且其5年生存率与预后率均处于较低水平。
方法：本研究选用黑色素瘤细胞系A375与G361作为体外实验模型。通过免疫组织化学（immunohistochemical, IHC）分析与生物信息学挖掘，证实PCDH9对黑色素瘤存在抑制作用。采用慢病毒介导PCDH9的敲低与过表达。通过检测细胞活力、细胞凋亡、细胞周期及划痕愈合实验，评估PCDH9表达改变对黑色素瘤细胞的影响。此外，还通过聚合酶链式反应（PCR）检测了PCDH9与MMP2、MMP9、CCND1及RAC1等基因的表达相关性。
结果：PCDH9的表达改变与MMP2、MMP9及RAC1的表达呈负相关，而与CCND1（细胞周期蛋白D1, Cyclin D1）及细胞凋亡呈正相关。上调PCDH9的表达可抑制黑色素瘤细胞增殖与迁移，但对细胞周期无显著影响。免疫组织化学结果显示，黑色素瘤组织中PCDH9的表达水平较低，且其主要定位于细胞质中。
结论：过表达PCDH9可抑制黑色素瘤细胞的恶性表型，PCDH9可作为黑色素瘤的独立预后因子；重新表达PCDH9可作为黑色素瘤治疗的潜在治疗策略。