Biological parameters of Amblyomma coelebs Neumann, 1906 (Acari: Ixodidae) under experimental conditions

Abstract One generation of Amblyomma coelebs life cycle under experimental conditions was evaluated. Ten tick pairs were allowed to feed on rabbits under laboratory conditions (LC), resulting six engorged females with a mean weight of 1,403.9 mg. Two females were maintained in a forest reserve under natural conditions (NC), and four were maintained in incubators (LC). The engorgement period lasted 10.33 days. Pre-oviposition periods were 10.75 (NC) and 22 days (LC). The mean egg-mass weight was 514.76 mg, and the blood meal conversion index was 36.67% (LC). Incubation period under NC and LC were 91 and 56.33 days and hatching rates were 50% and 28.33%, respectively. Larval engorgement period ranged from 4 to 10 days, with average weight of 1.1 mg. Engorged larvae were incubated under NC and LC, with a premolt period of 27 to 36 days and molting rate of 7.1% and 28.7%, respectively. Nymphal engorgement period ranged from 5 to 7 days, with a mean weight of 18.8 mg and a recovery rate of 54.54%. In LC, the ecdysis mean period was 24.5 days, and molting rate was 44.44%, resulting in 24 adult A. coelebs. Our results show a life cycle of 187.45 (NC) and 149 (LC) days.

摘要 本研究评估了实验条件下疣钝眼蜱（Amblyomma coelebs）的一代生活周期。将10对蜱虫置于实验室条件（LC）下的家兔体表吸血，最终获得6只饱血雌蜱，平均体重为1403.9 mg。其中2只雌蜱置于森林保护区的自然条件（NC）下饲养，剩余4只置于恒温培养箱内的实验室条件（LC）下饲养。饱血时长为10.33天。产卵前期在自然条件（NC）下为10.75天，实验室条件（LC）下为22天。卵块平均重量为514.76 mg，实验室条件（LC）下的血餐转化率为36.67%。自然条件（NC）与实验室条件（LC）下的孵化期分别为91天与56.33天，孵化率分别为50%与28.33%。幼虫饱血时长为4~10天，平均体重为1.1 mg。饱血幼虫分别置于自然条件（NC）与实验室条件（LC）下孵化，前蜕皮期为27~36天，蜕皮率分别为7.1%与28.7%。若虫饱血时长为5~7天，平均体重为18.8 mg，恢复率为54.54%。在实验室条件（LC）下，若虫蜕皮平均时长为24.5天，蜕皮率为44.44%，最终获得24只成体疣钝眼蜱。本研究结果显示，该蜱虫的完整生活周期在自然条件（NC）下为187.45天，实验室条件（LC）下为149天。