Alginate oligosaccharides forestalling intestinal inflammatory injury by modulating mucin glycosylation

Alginate oligosaccharides (AOS), marine-derived bioactive oligosaccharides, have been reported to protect intestinal barrier function, yet its effects on intestinal inflammation and the mechanisms remain unclear. In this study, the anti-inflammatory and its mechanism of AOS were investigated in lipopolysaccharide-induced inflammatory injury mice. Forty-eight 6-week-old C57BL/6J male mice were randomly allocated to four treatments: control group (CON), AOS group (AOS), model group (LPS), and AOS prevention group (AOS+LPS). The CON and LPS groups received drinking water, whereas the AOS and AOS+LPS groups received water supplemented with AOS. On day 21, mice in the LPS and AOS+LPS groups were intraperitoneally injected with LPS 6 h before sampling. 16s rDNA sequencing revealed that AOS reshaped the colonic microbiota.

褐藻寡糖（Alginate oligosaccharides, AOS）是一类源自海洋的生物活性寡糖，已有研究证实其可保护肠道屏障功能，但其对肠道炎症的作用及具体调控机制仍尚不明确。本研究以脂多糖（lipopolysaccharide, LPS）诱导的炎症损伤小鼠为模型，探究了AOS的抗炎作用及其潜在机制。实验选取48只6周龄雄性C57BL/6J小鼠，随机分为四组：对照组（CON）、AOS组（AOS）、模型组（LPS）以及AOS预防组（AOS+LPS）。对照组与模型组小鼠仅饮用普通饮水，AOS组及AOS预防组小鼠则饮用添加了AOS的饮水。实验第21天，模型组与AOS预防组小鼠于采样前6小时经腹腔注射脂多糖。16S rDNA测序结果显示，AOS可重塑小鼠结肠微生物群。