Expression data of whole genome from new diagnostic AML patients (with or without EMI) and healthy volunteers

Whole Genome microarray was conducted to further explore the underlying gene expression alterations in extramedullary infiltration (EMI) of AML. 4 matched samples from EMI, non-EMI AML patients and healthy volunteers were selected. Mononuclear cells from 12 bone marrow samples were separated according to protocols. RNA was then extracted and hybridization for further microarray analysis. EMI-related genes were selected by comparing with non-EMI AML patients and healthy controls. 4 independent experiments were performed using different donors for each experiment.

本研究采用全基因组微阵列（Whole Genome microarray）技术，旨在深入探究急性髓系白血病（AML）髓外浸润（extramedullary infiltration, EMI）中潜在的基因表达异常。研究选取了来自髓外浸润（EMI）AML患者、非髓外浸润（non-EMI）AML患者及健康志愿者的4份匹配样本。按照既定实验流程，从12份骨髓样本中分离得到单个核细胞。随后提取总RNA并完成杂交，用于后续的微阵列分析。通过与非髓外浸润AML患者及健康对照人群进行比对，筛选出与髓外浸润相关的差异基因。本研究共开展4次独立实验，每次实验均采用不同的供体样本。