Transcriptome of 3D cultivated colon cancer cell lines (HCT116, SW620, DLD1) and HCEC-1CT

The study aimed to identify transcripts of the protein-coding genes that could have regulatory role potentially relevant for malignant transformation of the gut mucosa and colon cancer onset and progression. The set of colon cancer cell lines (HCT116, SW620 and DLD1) and immortalized cells derived from healthy gut mucosa (HCEC-1CT) were cultivated in 3D and their transcriptomes were analyzed by RNA sequencing to profile expression for both coding and non-coding transcripts. Overall design: We performed transcript expression profiling of 3 colorectal cancer cell lines and HCEC-1CT cell line cultivated in 3D using RNA sequencing.

本研究旨在鉴定编码蛋白基因的转录本，此类转录本或可发挥潜在调控作用，其与肠黏膜恶性转化及结肠癌的发生、进展密切相关。
本研究选取结肠癌细胞系（HCT116、SW620及DLD1）与源自健康肠黏膜的永生化细胞系（HCEC-1CT），采用三维培养方式进行培养，并通过RNA测序（RNA sequencing）分析其转录组，以表征编码与非编码转录本的表达谱。
整体实验设计：本研究通过RNA测序，对3株结肠癌细胞系及HCEC-1CT细胞系的三维培养样本开展了转录本表达谱分析。