Prostaglandin E2 is required for skeletal muscle stem cell function, augmenting regeneration and strength

To identify the downstream signaling pathways that contribute to expansion of MuSCs by PGE2 Overall design: Muscle stem cells isolated from 2-4 month old mice by alpha7+/CD34+ sorting. Cells were treated in culture for 24 hours with DMSO or prostagland in E2 in biological triplicates. RNA was collected from 5,000-10,000 total cells.研究目的：明确前列腺素E2（Prostaglandin E2, PGE2）介导肌肉干细胞（Muscle Stem Cells, MuSCs）扩增的下游信号通路。 整体实验设计：采用α7+/CD34+分选法从2~4月龄小鼠体内分离肌肉干细胞。将细胞分为两组，分别以二甲基亚砜（Dimethyl sulfoxide, DMSO）及前列腺素E2进行体外处理，每组设置3次生物学重复，培养24小时后，从5000~10000个总细胞中提取RNA。