Epigenetic modules governing skin epidermal homeostasis [neonate skin epidermis]. Epigenetic modules governing skin epidermal homeostasis [neonate skin epidermis]

Skin homeostasis is guided by spatiotemporal regulation of gene expression, establishing each stage of keratinocyte differentiation. The current study presents transcriptomic and chromatin profiling for the proliferating (basal) and differentiated (suprabasal) cell populations derived from neonate mice (p0-p2) skin epidermis. This multi-omic approach will enable idenitification of cell specific epidermal cross-talk central to the equlibirum. Overall design: Transcriptomic profiling for basal and suprabasal cells in mouse neonate skin epidermis (Fvb/N strain and Dlx3 transgenic mice)

皮肤稳态（skin homeostasis）受基因表达的时空调控所引导，并由此确立角质形成细胞（keratinocyte）分化的各个阶段。本研究针对源自新生小鼠（出生后0~2日龄，p0-p2）皮肤表皮的增殖性（基底层）与分化性（基上层）细胞群，开展了转录组学与染色质谱分析。该多组学方法将有助于鉴定对表皮稳态平衡至关重要的细胞特异性表皮串扰。实验整体设计：对新生小鼠皮肤表皮的基底层与基上层细胞进行转录组学分析，实验对象涵盖Fvb/N品系小鼠与Dlx3转基因小鼠。