Supplementary file 1_Changes of potential shorty-chain fatty acids producing bacteria in the gut of patients with spinal cord injury: a systematic review and meta-analysis.zip

Gut bacteria that potential produce short-chain fatty acids (SCFAs) influences the recovery of motor function in the host in patients with spinal cord injury (SCI). We aimed to conduct a review and meta-analysis of the literature on gut microbiota in SCI patients. Following the Preferred Reporting Project for Systematic Review and Meta-Analysis (PRISMA), we searched Embase, PubMed, Cochrane Library, Web of Science (WOS) and ClinicalTrials.gov. The search period was from inception to March 31, 2024. We reported standardized mean differences (d) with 95% confidence intervals (CI) and used funnel plots and Egger tests to assess publication bias. The subacute of SCI data set revealed the microflora changes in the subacute phase, and meta-analysis summarized the changes in the chronic phase. Eleven studies (720 participants) were included, 2 phyla, 1 order, and 14 genus meta-analyses performed. No substantial heterogeneity was observed, and significant publication bias was not found among the studies included. In the subacute phase of spinal cord injury, the relative abundance of Bacteroidetes, Clostridiales, Faecalbacterium, Ruminococcus, Coprococcus, Lachnospira, Dorea, Prevotella, Roseburia, Atopobium, Bifidobacterium, Bacteroides, and Blautia increased. Firmicutes and Lactobacillus decreased. In the chronic phase, Firmicutes decreased in the SCI group. Bifidobacterium, Bacteroides, Blautia, and Eubacterium were found to have a higher average proportion of abundance in patients with SCI compared to non-SCI persons, and Clostridiales, Ruminococcus, Faecalbacterium, Coprococcus, and Lachnospira showed a lower relative abundance in SCI. The genus of potential SCFAs-producing bacteria is lower in the chronic phase of spinal cord injury than in the subacute phase, and gut dysbiosis is present in both the subacute and chronic phases.

潜在产短链脂肪酸（short-chain fatty acids, SCFAs）的肠道菌群，可影响脊髓损伤（spinal cord injury, SCI）患者宿主的运动功能恢复。本研究旨在针对脊髓损伤患者的肠道菌群相关文献开展系统综述与荟萃分析。本研究遵循《系统综述与荟萃分析首选报告项目》（Preferred Reporting Project for Systematic Review and Meta-Analysis, PRISMA），检索了Embase、PubMed、Cochrane图书馆、Web of Science（WOS）以及ClinicalTrials.gov数据库，检索时限为建库至2024年3月31日。本研究报告了标准化均数差（standardized mean difference, d）及95%置信区间（confidence interval, CI），并采用漏斗图与Egger检验评估发表偏倚。脊髓损伤亚急性期数据集揭示了亚急性阶段的菌群变化，而荟萃分析则总结了慢性阶段的菌群改变。最终纳入11项研究（共720名受试者），完成了2个菌门、1个菌目及14个菌属的荟萃分析。纳入研究未观察到显著异质性，且未发现明显的发表偏倚。在脊髓损伤亚急性期，拟杆菌门（Bacteroidetes）、梭菌目（Clostridiales）、粪杆菌属（Faecalbacterium）、瘤胃球菌属（Ruminococcus）、粪球菌属（Coprococcus）、毛螺菌属（Lachnospira）、多雷菌属（Dorea）、普雷沃菌属（Prevotella）、罗氏菌属（Roseburia）、隐秘杆菌属（Atopobium）、双歧杆菌属（Bifidobacterium）、拟杆菌属（Bacteroides）以及布劳特氏菌属（Blautia）的相对丰度显著升高；厚壁菌门（Firmicutes）与乳杆菌属（Lactobacillus）的相对丰度则显著降低。在慢性期，脊髓损伤组的厚壁菌门相对丰度有所降低。与非脊髓损伤人群相比，脊髓损伤患者的双歧杆菌属、拟杆菌属、布劳特氏菌属以及真杆菌属（Eubacterium）的平均相对丰度更高；而梭菌目、瘤胃球菌属、粪杆菌属、粪球菌属以及毛螺菌属的相对丰度在脊髓损伤患者中更低。潜在产短链脂肪酸的菌属在脊髓损伤慢性期的相对丰度低于亚急性期，且亚急性与慢性阶段均存在肠道菌群失调。