Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2

Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels.

海洋酸化（Ocean acidification, OA）已被证实会影响软体动物的一系列正常生理过程，尤其对其贝壳的形成过程构成重大威胁。本研究采用整合代谢组学（metabolomics）与蛋白质组学（proteomics）的方法，探究了高pCO₂条件暴露对长牡蛎（Crassostrea gigas）外套膜组织的影响。代谢组与蛋白质组分析结果显示，高pCO₂会影响长牡蛎的能量代谢，其特征为三磷酸腺苷（ATP）、琥珀酸（succinate）、苹果酸脱氢酶（MDH）、磷酸烯醇式丙酮酸羧激酶（PEPCK）及乙醛脱氢酶（ALDH）水平发生差异性改变。此外，钙调蛋白-2（calponin-2）、原肌球蛋白（tropomyosins）及肌球蛋白轻链（myosin light chains）的上调表明，高pCO₂可能导致长牡蛎外套膜组织的细胞骨架结构紊乱。本研究表明，蛋白质组学与代谢组学的联合应用可为从分子层面解析海洋酸化的影响提供重要启示。