c-Myc binding in HL60 cells

Analysis of technical variance of ChIP-on-Chip studies by characterization of Myc-binding in HL60 cells. Keywords: Chip-on-chip Characterization of c-Myc binding in HL60 cells. Thirteen biologically independent replicates of growing HL60 cells were subjected to ChIP with an N-terminal c-Myc antibody and hybridized to CpG island microarrays

本研究通过表征HL60细胞中的c-Myc结合特征，分析染色质免疫沉淀芯片（ChIP-on-Chip）实验的技术差异。关键词：染色质免疫沉淀芯片（ChIP-on-Chip）；HL60细胞中c-Myc结合的表征。将13份独立生物学重复的增殖期HL60细胞采用N端c-Myc抗体进行染色质免疫沉淀（ChIP），并将所得产物与CpG岛微阵列（CpG island microarrays）进行杂交。