The effect of simulated night shift work on the circadian regulation of the human transcriptome

Eight healthy human subjects were enrolled in a 6-day simulated shift work protocol. Blood samples were collected during the two 24-hour measurement periods. Blood samples were collected every 4 hours during both measurement periods. Subjects entered the lab on Day 1. At the start of Day 2, the first 24-hour measurement period was started. Subjects slept according to their habitual sleep/wake schedule, followed by a 16-hour constant posture procedure. On days 3-6, the sleep period was delayed by 10 hours. Following the third night on this schedule, subjects underwent another 24-hour measurement period. During both measurement periods, 7 blood samples were collected and PBMCs were isolated. mRNA was extracted, labelled, and hybridized to microarrays. A total of 103 samples were collected from 8 subjects spread across two 24-hour measurement periods

招募了8名健康人类受试者参与一项为期6天的模拟轮班工作方案。研究设置两个24小时测量周期，每个周期内均以每4小时一次的频率采集血液样本。受试者于第1天进入实验室，第2天伊始启动第一个24小时测量周期：受试者按照自身日常睡眠-觉醒节律作息，随后需完成16小时的固定姿势维持流程。第3至6天，受试者的睡眠时段延后10小时；在遵循该作息方案度过第三个夜晚后，受试者进入第二个24小时测量周期。两个测量周期内均采集7份血液样本，并分离外周血单个核细胞（Peripheral Blood Mononuclear Cells，PBMCs）。随后提取信使核糖核酸（mRNA）并进行标记，将标记后的产物与基因芯片（microarrays）进行杂交。本次研究共从参与两个24小时测量周期的8名受试者身上，总计采集到103份样本。