miRNA in HUVEC culture supernatant

Human umbilical vein endothelial cells (HUVEC) were cultured in serum-free medium with 50 μmol/L ADMA (ADMA group) or without ADMA (NA group ). Asymmetric dimethylarginine is a typical uremic toxin which used to induce HUVEC injury. ADMA treated HUVEC may release different miRNAs campared to normal control cells. The cells culture supernatant were collected and detected.

将人脐静脉内皮细胞（Human umbilical vein endothelial cells，HUVEC）分为两组培养：一组置于含50 μmol/L 不对称二甲基精氨酸（Asymmetric dimethylarginine，ADMA）的无血清培养基中（ADMA组），另一组置于不含ADMA的无血清培养基中（NA组）。不对称二甲基精氨酸是典型的尿毒症毒素，可诱导人脐静脉内皮细胞损伤。相较于正常对照细胞，经ADMA处理的HUVEC可能会释放差异表达的微小核糖核酸（microRNA，miRNA）。收集各组细胞培养上清液并进行检测。