Crustacean leg regeneration restores cell diversity

Animals can regenerate complex organs, yet this frequently results in imprecise replicas of the original structure. In the crustacean Parhyale, we examine the fidelity of leg regeneration using cellular composition and cell molecular profiles. Single-nuclei sequencing shows that regenerated and uninjured legs are indistinguishable in terms of cell type composition and transcriptional profiles. Overall design: Legs of the Crustacean Parhyale hawaiensis were amputated and analyzed through scRNAseq after regeneration.

动物具备再生复杂器官的能力，但该过程往往无法生成与原始结构完全一致的精准复制品。本研究以甲壳类动物（crustacean）副跳虾属（Parhyale）为研究对象，通过细胞组成与细胞分子谱分析，探究其步足再生的保真度。单细胞核测序（single-nuclei sequencing）结果显示，再生步足与未损伤步足在细胞类型组成及转录组谱层面无显著差异。
总体实验设计：对夏威夷副跳虾（Parhyale hawaiensis）的步足进行截肢操作，待其完成再生后，通过单细胞RNA测序（scRNAseq）开展分析。