Chemical-based external stimulation reprograms human somatic cells into pluripotency (ATAC-seq)

Here, we report that chemical-based external stimulation reprograms human somatic cells to generate human induced pluripotent stem cells. Using transcriptome, chromatin accessibility and DNA methylome profiling, we identified the cells exhibited key features of embryonic stem cells in transcriptomic profiles, epigenetic status. In addition, we also analyzed the reprogramming process. The induced PSCs were analyzed and compared to human somatic cells and human embryonic stem cells (hES) by ATAC-seq.

本研究证实，借助基于化学试剂的外源刺激，可将人类体细胞重编程为人类诱导多能干细胞（human induced pluripotent stem cells）。本研究通过转录组（transcriptome）、染色质可及性（chromatin accessibility）与DNA甲基化组（DNA methylome）分析，证实所获得的细胞在转录组特征与表观遗传状态上均具备胚胎干细胞的核心特征。此外，本研究还对重编程过程进行了系统分析；通过转座酶可及性测序（ATAC-seq），对诱导获得的多能干细胞、人类体细胞与人类胚胎干细胞（human embryonic stem cells，hES）开展了对比分析。