GSC differentiation-reversion test. Drosophila melanogaster

gene expression is tested for an association with GSCs by comparing expression levels in ovary tips of c587-GAL4; hs-bam UAS-dpp females at 0 hr, 20 hr and 50 hr after heat shock ; results of two independent experiments are given as expt 1 and expt 2 Keywords: time course, heat shock, bam overexpression Overall design: 0 hr ovary tips contain many GSCs 20 hr hs-bam has induced differentiation of all GSCs 50 hr Bam has turned over and cystocytes have reverted to become GSCs again see Kai et al. (2005) Dev. Biol.

本数据集通过对比c587-GAL4; hs-bam UAS-dpp雌性果蝇热激后0小时、20小时及50小时的卵巢尖端基因表达水平，检测基因表达与生殖系干细胞（Germline Stem Cells, GSCs）的相关性。两项独立重复实验的结果分别记为实验1（expt 1）与实验2（expt 2）。关键词：时间进程、热激、bam基因过表达。实验整体设计：热激后0小时的卵巢尖端含有大量生殖系干细胞；热激20小时后，hs-bam诱导所有生殖系干细胞发生分化；热激50小时后，Bam蛋白已完成降解周转，包囊细胞（cystocytes）逆转为生殖系干细胞。相关细节参见Kai等人（2005年）发表于《发育生物学（Dev. Biol.）》的研究。