Pol-IV dependent transcripts in Arabidopsis. Arabidopsis thaliana

small RNA, PATH and mRNA libraries were made to characterize Col and various small RNA biogenesis mutants. Overall design: Genome-wide RNA profiling was done by Illumina TruSeq sample preparation kits followed by high-throughput sequencing with Illumina HiSeq 2500 platform.

本研究为表征哥伦比亚生态型（Col）及各类小分子RNA生物发生突变体，构建了小分子RNA（small RNA）、PATH及信使RNA（mRNA）文库。实验整体设计：采用Illumina TruSeq样本制备试剂盒开展全基因组RNA谱分析，随后依托Illumina HiSeq 2500平台完成高通量测序。