10X genomic single cell RNA-seq of WT and p47phox-KI lungs with acute lung injury

We performed single cell RNA sequencing of CD45-negative cells sorted from WT and p47phox-KI lungs with ALI to characterize the impacts of the loss of p47phox phosphorylation in blood cells on pulmonary endothelial and epithelial cells. We found many genes are pertinent to enhancement of pulmonary vasculature integrity, maintain alveolar roles, promote ATII cell proliferation and lower oxidative stress state in p47phox-KI lungs. Compare gene expression in single cell level between WT and p47phox-KI lungs

本研究对伴有急性肺损伤（Acute Lung Injury, ALI）的野生型（Wild Type, WT）及p47phox敲入（p47phox Knock-In, p47phox-KI）小鼠肺组织中分选得到的CD45阴性细胞开展单细胞RNA测序（single cell RNA sequencing, scRNA-seq），以解析血细胞中p47phox磷酸化缺失对肺血管内皮细胞与肺泡上皮细胞的影响。研究发现，在p47phox-KI小鼠肺组织内，诸多基因与肺血管屏障完整性增强、肺泡功能维持、II型肺泡上皮细胞（Alveolar Type II epithelial cell, ATII）增殖促进以及氧化应激水平降低密切相关。本数据集旨在比较野生型与p47phox-KI小鼠肺组织在单细胞水平的基因表达差异。