Unfractionated (>0.2 uM) seawater samples from GEOTRACES GA03 transect Targeted loci environmental. Unfractionated (>0.2 uM) seawater samples from GEOTRACES GA03 transect

Amplicon sequences from GEOTRACES cruise GA03 generated with 515Y/926R primers (Parada et al., 2016) with the same DNA used to produce metagenomes described by Biller et al 2018 (see link to paper for more information and accessions for corresponding metagenomes). Note that these samples come from unfractionated seawater (>0.2uM) and thus are "whole-seawater" samples. In addition, the primers used target both 16S and 18S, and thus require a specialized protocol to separate 16S sequences (which overlap) and 18S sequences (which do not). A suggested workflow is provided in the github link below. Data processed with DADA2 into amplicon sequence variants will be available on the Simons Collaborative Marine Atlas Project (CMAP) at a later date.

本数据集包含来自全球海洋示踪物计划（GEOTRACES）GA03航次的扩增子序列（amplicon sequences），该序列通过515Y/926R引物（Parada等，2016）扩增得到，所用DNA与Biller等人2018年发表的宏基因组研究所使用的DNA完全一致；如需获取更多细节及对应宏基因组的登录号，请参阅论文链接。需注意，本数据集的样本取自未分级海水（孔径>0.2μm），因此属于“全海水”样本。此外，本次使用的引物可同时靶向16S与18S序列，因此需要采用专属实验流程以分离存在序列重叠的16S扩增子序列与无序列重叠的18S扩增子序列。下文附带的GitHub链接中提供了推荐的分析流程。经DADA2工具处理得到扩增子序列变异体（amplicon sequence variants, ASV）的数据集，将于后续在西蒙斯海洋协作地图集项目（Simons Collaborative Marine Atlas Project, CMAP）平台上线发布。