Fig3G_RACE_luc-GCTG_R1_LG129.sgd

HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with PA-X from the influenza A/Puerto Rico/8/1984 (H1N1) or vector, and luciferase reporters with or without 99 bp repeated sequence or an additional GCTG tetramer. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.

将HEK293T ishXrn1细胞经多西环素处理3至4天以诱导Xrn1基因敲低，随后分别转染甲型流感病毒A/Puerto Rico/8/1984（H1N1）来源的PA-X蛋白或空载体，以及带有99bp重复序列、不带有99bp重复序列或额外带有GCTG四聚体的荧光素酶报告基因。24小时后收集RNA，采用结合荧光素酶序列的引物通过5' 末端快速扩增（5' RACE）技术进行分析。