Data_Sheet_2_Integrative proteomics and metabolomics of Guizhou Miao Sour Soup affecting simple obese rats.XLSX

Miao Sour Soup (MSS) is a fermented product from the Qiandongnan region of Guizhou Province, which enrich many beneficial ingredients and is widely consumed in the whole China. Fermented food is beneficial to physical health with the potential positive regulating affection on simple obesity. In this study, we analyzed the mechanism of action of MSS to prevent simple obesity induced by high-fat diet by proteomics and metabolomics. Quantitative proteomics with tandem mass tagging labeling and liquid chromatography-mass spectrometry was used to analyze the changes of liver proteins and metabolites after the MSS intervention. MSS intervention upregulated 33 proteins and 9 metabolites and downregulated 19 proteins and 10 metabolites. Bioinformatics analysis showed that MSS could prevent simple obesity by acting on the PPAR signaling pathway, retinol metabolism, fatty acid β-oxidation, fatty acid degradation, fatty acid biosynthesis, glycine, serine and threonine metabolism, pyruvate metabolism, citrate cycle (TCA cycle) and other signaling pathways. This study provides new insights into the use of MSS to prevent simple obesity caused by high-fat diets and the search for healthy eating patterns with MSS.

苗家酸汤（Miao Sour Soup, MSS）是产自贵州省黔东南地区的发酵食品，其富含多种有益功效成分，目前已在全国范围内广泛食用。发酵食品有益于身体健康，对单纯性肥胖具有潜在的正向调节作用。本研究采用蛋白质组学与代谢组学技术，解析了苗家酸汤干预高脂饮食诱导单纯性肥胖的作用机制。本研究通过串联质量标签标记联合液相色谱-质谱联用的定量蛋白质组学方法，分析了苗家酸汤干预后肝脏组织的蛋白质与代谢物表达变化。经苗家酸汤干预后，共有33种蛋白质与9种代谢物表达上调，19种蛋白质与10种代谢物表达下调。生物信息学分析结果显示，苗家酸汤可通过调控过氧化物酶体增殖物激活受体（PPAR signaling pathway）信号通路、视黄醇代谢、脂肪酸β-氧化、脂肪酸降解、脂肪酸生物合成、甘氨酸、丝氨酸与苏氨酸代谢、丙酮酸代谢、三羧酸循环（TCA cycle）等通路发挥预防单纯性肥胖的作用。本研究为利用苗家酸汤预防高脂饮食诱导的单纯性肥胖，以及探索基于苗家酸汤的健康饮食模式提供了全新的研究视角。