Effect of a PDD on oxaliplatin-induced mechanical and cold hypersensitivity.

Rats were fed with a normal diet (triangles) or a PDD (circles) for 7 days before the beginning of the tests and through to the end of the experiment. At day 0, after measuring baseline mechanical pain thresholds, rats were injected with oxaliplatin (6 mg/kg, i.p., OXA, black symbols) or vehicle (CT, white symbols). (A) Mechanical pain hypersensitivity expressed as paw withdrawal threshold (gram). (B) Cold hypersensitivity (12°C vs. 25°C) expressed as time spent on the cold plate (%). Two-way repeated-measures ANOVA followed by a Tukey-Kramer post hoc test. *, pvs. normal diet - CT; ¤¤, p¤¤¤, pvs. PDD - OXA. All data are expressed as means ± SEM (n = 6 to 12 per group).

在实验开始前7天直至整个实验结束期间，大鼠分别饲喂正常饲料（标记为三角形）或PDD饲料（标记为圆形）。实验第0天，在测定基础机械痛阈值后，大鼠分别腹腔注射奥沙利铂（oxaliplatin，6 mg/kg，下称OXA，黑色标记）或溶媒（vehicle，下称CT，白色标记）。(A) 机械痛觉超敏以缩爪阈值（单位：克）表示；(B) 冷痛觉超敏以冷板停留时间占比（%）表示，测试采用12℃与25℃温度对比。统计分析采用双因素重复测量方差分析（two-way repeated-measures ANOVA），后续辅以Tukey-Kramer事后检验。显著性标记说明：*表示与正常饲料-溶媒组（normal diet - CT）相比存在统计学差异；¤¤、¤¤¤表示与PDD饲料-奥沙利铂组（PDD - OXA）相比存在统计学差异。所有数据以平均值±标准误（standard error of the mean, SEM）表示，每组样本量为6~12只。