Expression profiling of lung endothelial cells during metastatic progression. [RNA-Seq]. Expression profiling of lung endothelial cells during metastatic progression. [RNA-Seq]

Lung EC were isolated at sequential stages of tumor progression and bulk RNA-seq analysis was performed. Overall design: Total RNA-sequencing FACS-sorted lung endothelial cells was undertaken. Four independent biological replicates for each timepoint were analysed.

研究人员在肿瘤进展的连续时序阶段分离得到肺内皮细胞（Lung EC），并开展了批量RNA测序（bulk RNA-seq）分析。实验整体设计：本研究对经荧光激活细胞分选（FACS）纯化的肺内皮细胞进行总RNA测序，每个时间点均设置四个独立生物学重复并完成测序分析。